With increased water testing requirements due to the U.S. Produce Safety Rule and third-party audits, more farms are submitting water samples to third-party labs for microbial analysis. This brief video demonstrates the best practices for collecting a water sample from a pond or other irrigation source, including the sample type needed by a produce grower for their microbial water quality profile for the Produce Safety Rule. The authors describe when a sample may be needed, where to collect the sample, and proper sample handling during and after collection.